Cyclop-eeze Nutrition Reports

Introduction

Cyclop-eeze are derived from the family of copepod grown in a pristine, arctic salina in the north of Canada. Cyclop-eeze thrive during the short spring and summertime season. They forage on Spirulina rich in the highly unsaturated fatty acids (HUFA’s) and biological pigments. More importantly, the Cyclop-eeze are busy synthesizers of HUFA’s, especially the important omega (w-3) variety. Our previous research showed that the pigments were simply accumulated by Cyclop-eeze in proportion to its abundance in their diets. The pigments protected Cyclop-eeze from harmful photodynamic effects of visible lights. Our research also showed that cold water temperature contributed positively to the brilliant orange color and active behavior of Cyclop-eeze. The special salt lake (pH 9.7, Salinity 72 ppt) where Cyclop-eeze grow is unique as it is very rich in a wide variety of inorganic chemicals, several of which are important elements in specific needed for the production of HUFA’s and biological pigments. Upon harvest, the Cyclop-eeze are immediately deep frozen so that the important nutrients are retained. Shortly thereafter, the frozen blocks are taken to the Argent plant for freeze drying (lyophilization).

Analytical Method

Fatty Acid Profile : American Organization of Analytical Chemistry (AOAC) Method 969.33

Free Amino Acid:  AOAC Method 982.30, Section D & F

Mineral Profile:  Freeze-dried Cyclop-eeze were minced in a grinder, and whole Cyclop- eeze dry weights were determined by drying to a constant weight at 105oC. After drying, the samples were charred and then incinerated at 550oC for 16 h. The ash was dissolved in a mixture of equal parts of concentrated HCl and HNO3, and appropriately diluted within the analytical capabilities of the ICP-MS.(Jarrelll-Ash Atom, Fisher Scientific, Waltham, MA).

Carotenoid Profile: Chromatography: An aliquot of sample (2.5 g) was extracted in Acetone (2 times, soak, grind, soak) and the extract cleared by filtration through a glass fiber through a glass fiber filter. The extract was analyzed by reverse-phase HPLC (Varian Microsorb C18 MV) and the peaks were integrated at 480 nm.

Mass-Spectroscopy: a concentrated sample was injected on the rp-HPLC system and the carotenoids peaks were collected and subjected to mass-spectroscopy on a Finigan LCQ to confirm. The fragmentation patterns are consistent with the structure of correstponding carotenoids.

UV/Vis Spectrometer: A 1 g sample of Cyclopee was extracted in 30 ml acetone by sonication (2x5 min) in an ice bath. The particulate matter was centrifuged off. The absorbance of the extract was measured at 481 nm to calculated the carotenoid concentration of the Cyclopee using an extinction coefficient of E (1%, 1cm)=2100 (UNESCO handbook).

Results

Cyclop-eeze are very rich in fatty acid (35%), especially the importand fatty acid EPA(11.74%) and DHA (11.09%). By comparing with Artemia, Cyclop-eezee appear to be a very good source of n3 (w-3) fatty acids and the fatty acid profile of Cyclop-eezee is similar to that of menhaden fish oil used in dish diets. Cyclop-eeze are also rich in the 17 essential amino acids and minerals.

The carotenoid data show that Cyclopee have very high astaxanthin concentration comparared with Artemia. (A comparison between Cyclop-eeze and Artemia was shown in Table 1.) Cyclop-eeze have been successfully used for pigmenting shrimp, rainbow trout, tropicals (Marine and Fresh), etc. in many test feed around the world. 94% of the carotenoid of Cyclop-eeze is astaxanthin, which imparts the red hue to aquatic animals. The astaxanthin accumulated in the skin of samon and ornamental fish is mostly an esterified form of the carotenoid. Test feed reports of Cyclopee showed that daitary astaxanthin esters result in pigment depositions several times higher than either freee astaxanthin or other piment sources. Enhanced coloration effect (2%-5%) is very evident through thest studies. Growth enhancement is 15%-30%, and reporductive enhancement is 5%-15%.

Table 1 Cyclop-eeze and Artemia Nutrients Comparison

Parameters Cyclop-eeze Artemia
Protein 60 73
Lipid 35 18
Ash 3 5
Carbonhydrate 2 3
Astaxanthin 2867 ppm <D.L
Canthaxanthin 15 ppm 102 ppm
18:3n3 (Linolenic Acid) 10.45% 1.3%
20:5n3 (EPA) 11.74% 0.25
22:6n3 (DHA) 11.09% 2.7%
Enzyme Activity

(Superoxide Dismutase)

30,000 22,500
Average Body Length 800 um 400 um

Note: Cyclop-eeze Sample: Argent #70280

Artemia Sample: Argent #95-p Argentemia Platinum Label

Our preliminary study showed that Cyclop-eezee have also abudant immunochemicals, viatamins attractants, and other precious natural pigments. Active undergoing research is being conducted to explore those areas.

Summary

Cyclop-eeze are biologically engineered and cultivated organism, with much higher concentration of HUFA’s and biopigments than Artemia and can be an ideal substitute of Artemia.

Cyclop-eeze can be widely used to feed all kinds of aquatic animals, especially, shrimp, ornamental fish, etc.

Cyclop-eeze can be an excellent color booster and immune stimulator for shrimp and fish.

Cyclop-eeze have potential immunization chemicals and strong attractants.

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