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MS-222

FINQUEL

FINQUEL is intended for the temporary immobilization of fish, amphibians, and other aquatic, cold-blooded animals. It has long been recognized as a valuable tool for the proper handling of these animals during manual spawning (fish stripping), weighing, measuring, marking, surgical operations, transport, photography, and research.

DIRECTIONS FOR USE ON FISH CONCENTRATIONS

FINQUEL is effective and safe for the anesthesia of fish when used as directed. Its use is governed by, and can be tailored to, the needs of individual fishery personnel. Sedation and various rates of anesthetization are controlled by the concentration. The versatility of FINQUEL is demonstrated by the fact that it has been used in fisheries at levels ranging from 10 to 1,000 mg./liter. The action of the anesthetic is slowed at cooler temperatures, in extremely soft water (approximately 10 mg./liter of CaCO3. or less), and in larger fish. Also, efficacy may vary with species. Thus, it is imperative that preliminary tests of anesthetic solution be made against small numbers of fish to determine the desired rates of anesthesia and exposure times for the specific lots of fish under prevailing conditions.

METHODS OF APPLICATION

1. General anesthesia: - For most situations where rapid or moderately rapid anesthesia is required, FINQUEL may be applied in a bath, i.e., the fish are immersed in the anesthetic solution. Containers may be of glass, plastic, steel, aluminum, or other suitable material. However, do not use galvanized or brass containers unlless treated or sealed to prevent dissolution of zinc. Size of container is determined by individual needs, but the fish should not be overcrowded. Discard anesthetic solutions when a loss in potency is noted, or when the solutions become fouled with mucus or excrement.

2. For surgery and certain physiologic studies, the fish may be anesthetized to loss of reflex, removed from the anesthetic, and then positioned so that the gills are bathed in a sedating concentration of FINQUEL. Some investigations have developed flowing, recirculating systems for bathing the gills with anesthetic during surgery.

Large fishes such as sharks and rays are anesthetized within minutes by spraying the gills with a 1 g./liter solution of FINQUEL. The application is made by means of a water pistol, bulb syringe, hand pump, etc.

3. Transport - FINQUEL has been used to sedate fish during transport. It is more successful in cold than in warm water, and it is instrumental in reducing injuries because of hyperactivity. Fish are usually transported by means of distribution units (tank trucks), or by air in plastic bags. In either case, the fish should be fasted before-hand to reduce metabolic wastes. Aslo, some workers suggest pretransport sedation for several hours to lower metabolism. With distribution units, the fish may be fasted and sedated prior to loading. The anesthetic solution is prepared in the distribution unit and oxygenated. Then, the fish are added and temperature acclimated.

In air shipments, the anesthetic solution is placed in a suitable plastic bag, the sedated fish are added, the bag inflated with oxygen, tied securely, and placed in a second bag. This bag is also tied, and then placed on ice in an insulated container. A modification of this method involveds complete anesthesia of the fish, and placing them in water bags which contain no anesthetic. In any case, upon arrival, the fish should be acclimated slowly to new environmental temperatures.